Asymmetric proteasome segregation as a mechanism for unequal partitioning of the transcription factor T-bet during T lymphocyte division.

نویسندگان

  • John T Chang
  • Maria L Ciocca
  • Ichiko Kinjyo
  • Vikram R Palanivel
  • Courtney E McClurkin
  • Caitlin S Dejong
  • Erin C Mooney
  • Jiyeon S Kim
  • Natalie C Steinel
  • Jane Oliaro
  • Catherine C Yin
  • Bogdan I Florea
  • Herman S Overkleeft
  • Leslie J Berg
  • Sarah M Russell
  • Gary A Koretzky
  • Martha S Jordan
  • Steven L Reiner
چکیده

Polarized segregation of proteins in T cells is thought to play a role in diverse cellular functions including signal transduction, migration, and directed secretion of cytokines. Persistence of this polarization can result in asymmetric segregation of fate-determining proteins during cell division, which may enable a T cell to generate diverse progeny. Here, we provide evidence that a lineage-determining transcription factor, T-bet, underwent asymmetric organization in activated T cells preparing to divide and that it was unequally partitioned into the two daughter cells. This unequal acquisition of T-bet appeared to result from its asymmetric destruction during mitosis by virtue of concomitant asymmetric segregation of the proteasome. These results suggest a mechanism by which a cell may unequally localize cellular activities during division, thereby imparting disparity in the abundance of cell fate regulators in the daughter cells.

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عنوان ژورنال:
  • Immunity

دوره 34 4  شماره 

صفحات  -

تاریخ انتشار 2011